Proteome analysis guided genetic engineering of Corynebacterium glutamicum S9114 for tween 40-triggered improvement in l-ornithine production
نویسندگان
چکیده
منابع مشابه
Systematic pathway engineering of Corynebacterium glutamicum S9114 for l-ornithine production
BACKGROUND L-Ornithine is a non-protein amino acid with extensive applications in medicine and the food industry. Currently, L-ornithine production is based on microbial fermentation, and few microbes are used for producing L-ornithine owing to unsatisfactory production titer. RESULTS In this study, Corynebacterium glutamicum S9114, a high glutamate-producing strain, was developed for L-ornit...
متن کاملMetabolic engineering of Corynebacterium glutamicum for L-serine production.
Although L-serine proceeds in just three steps from the glycolytic intermediate 3-phosphoglycerate, and as much as 8% of the carbon assimilated from glucose is directed via L-serine formation, previous attempts to obtain a strain producing L-serine from glucose have not been successful. We functionally identified the genes serC and serB from Corynebacterium glutamicum, coding for phosphoserine ...
متن کاملEngineering of Corynebacterium glutamicum for growth and production of L-ornithine, L-lysine, and lycopene from hexuronic acids
Background: Second-generation feedstocks such as lignocellulosic hydrolysates are more and more in the focus of sustainable biotechnological processes. Corynebacterium glutamicum, which is used in industrial amino acid production at a million-ton scale, has been engineered towards utilization of alternative carbon sources. As for other microorganisms, the focus has been set on the pentose sugar...
متن کاملEngineering Corynebacterium glutamicum for violacein hyper production
BACKGROUND Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications derived from L-tryptophan. RESULTS Corynebacterium glutamicum ATCC 21850 that co...
متن کاملMetabolic evolution of Corynebacterium glutamicum for increased production of L-ornithine
BACKGROUND L-ornithine is effective in the treatment of liver diseases and helps strengthen the heart. The commercial applications mean that efficient biotechnological production of L-ornithine has become increasingly necessary. Adaptive evolution strategies have been proven a feasible and efficient technique to achieve improved cellular properties without requiring metabolic or regulatory deta...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2020
ISSN: 1475-2859
DOI: 10.1186/s12934-019-1272-0